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Justin Daho Lee

Molecular Engineering PhD Student at University of Washington

Seattle, WA

I'm interested in developing protein sensors for various neuromodulators and optogenetic zebrafish model to uncover the complexity of excitatory/inhibitory balance in the pathogenesis of neurological disorders. #Protein Engineering #Neuroscience #Optogenetics #Genetically Encoded Sensors

Work Experience

  • Andre Berndt Lab

    Research Scientist|Apr, 2018 – Current

    Berndt lab is a molecular design lab which develops fluorescent biosensors for detecting biochemical signals in neuronal networks in real time. Our goal is to monitor the activity of neurotransmitter, neuromodulators, hormones, ions and intracellular signaling molecules in live tissue and behaving animals at high spatial and temporal resolution. We aim to use these tools to identify impaired network dynamics in animal models for neurological disorders which will close critical knowledge gaps in our understanding of diseases such as autism and epilepsy.

    • Optogenetic modeling and rescue of neuropathological brain states in zebrafish
    • High-throughput engineering of molecular sensors for brain function

  • NanoSurface Biomedical Inc.

    Cofounder|Mar, 2014 – Apr, 2018

    NanoSurface Biomedical(NSB) provides products and services for drug development, disease modeling, and fundamental research into cell biology. NanoSurface culture products imitate the native extracellular matrix, and structure cultured cells into physiologically relevant tissue models. NSB was spin-off from technologies developed in Deok-Ho Kim's Lab.

    • NanoSurface Biomedical brings nanotopography to various in vitro system to re- capitulate micro cellular environment that enables more ”physiological” cell/tissue culture.
    • As a head of technology, successfully launched first product line ”ANFS”
    • Transition from management role to consulting role to focus on MS degree(2016)
    • Academic collaboration to develop stretchable chamber with Extracellular Matrix mimicking nanotexture (2018)

  • Deok-Ho Kim Lab

    Student Researcher|Jan, 2012 – Apr, 2018

    Kim Lab's research spans the disciplinary boundaries between nanotechnology, biomaterials, and mechanobiology with an emphasis on their applications to tissue engineering and regenerative medicine. Through the use of multi-scale (nano/micro/meso) fabrication and integration tools, Kim Lab focus on the development and application of bio-inspired materials/devices and functional tissue engineering models for elucidating regenerative biology, drug screening, disease modeling, and stem cell-based therapies.

    • Combinatorial maturation of iPSC derived skeletal muscle cell - nanotopography and mechanical stretching
    • iPSC myogenic differentiation
    • Tissue engineered human neuromuscular junctions for study of CMT II pathophysiology
    • Graphene-based multifunctional nanomaterials for cardiac tissue and stem cell Engineering
    • Biomimetically nano-textured micro electrode array device for drug-induced cardiotoxicity screening
    • Biocompatible transparent substrate for corneal epithelial cell migration enhancement
    • Mathematical modeling of transfer of intercellular cytoplasmic proteins
    • Thermoresponsive nanofabricated substratum for the engineering of three dimensional tissues with layer-by-layer architectural control
    • Synergistic effects of topography and rigidity difference on human umbilical smooth muscle cell
    • Paid part-time research scientist from Jan. 2012 to Aug. 2012
    • Multiscale device fabrication manager
    • Mentored undergraduate students and volunteers

Education

  • University of Washington

    PhD - Molecular Engineering|Sep, 2018 – Current

  • University of Washington

    MS - Applied Bioengineering|Aug, 2016 – Dec, 2017

  • University of Washington

    BS - Physiology|Aug, 2010 – Jun, 2016

Projects

Skills

    • Molecular Cloning and PCR
    • scRT-PCR
    • Multiphoton Microscopy
    • Confocal Microscopy
    • Prototyping (3D printing, CNC, laser cutting)
    • Stem Cell Maintenance and Differentiation
    • Western Blotting
    • Immumnofluroscence Staining
    • ImageJ
    • Soft Lithography

Contact Information

    Email:
    justindaholee@gmail.com
    Telephone:
    2064198197
    Website:
    www.justindaholee.com