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Justin Daho Lee

Molecular Engineering PhD Student at University of Washington
Seattle, WA
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justindaholee.com
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justindaholee@gmail.com
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2064198197
I am a 4th year Ph.D. student at University of Washington building #Protein Engineering #Neuroscience #Optogenetics #Genetically Encoded Sensors
J
L

Work Experience

Andre Berndt Lab

Research Scientist
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Apr 2018 - Current

Berndt lab is a molecular design lab which develops fluorescent biosensors for detecting biochemical signals in neuronal networks in real time. Our goal is to monitor the activity of neurotransmitter, neuromodulators, hormones, ions and intracellular signaling molecules in live tissue and behaving animals at high spatial and temporal resolution. We aim to use these tools to identify impaired network dynamics in animal models for neurological disorders which will close critical knowledge gaps in our understanding of diseases such as autism and epilepsy.

  • Optogenetic modeling and rescue of neuropathological brain states in zebrafish
  • High-throughput engineering of molecular sensors for brain function

NanoSurface Biomedical Inc.

Cofounder
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Mar 2014 - Apr 2018

NanoSurface Biomedical(NSB) provides products and services for drug development, disease modeling, and fundamental research into cell biology. NanoSurface culture products imitate the native extracellular matrix, and structure cultured cells into physiologically relevant tissue models. NSB was spin-off from technologies developed in Deok-Ho Kim's Lab.

  • NanoSurface Biomedical brings nanotopography to various in vitro system to re- capitulate micro cellular environment that enables more ”physiological” cell/tissue culture.
  • As a head of technology, successfully launched first product line ”ANFS”
  • Transition from management role to consulting role to focus on MS degree(2016)
  • Academic collaboration to develop stretchable chamber with Extracellular Matrix mimicking nanotexture (2018)

Deok-Ho Kim Lab

Student Researcher
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Jan 2012 - Apr 2018

Kim Lab's research spans the disciplinary boundaries between nanotechnology, biomaterials, and mechanobiology with an emphasis on their applications to tissue engineering and regenerative medicine. Through the use of multi-scale (nano/micro/meso) fabrication and integration tools, Kim Lab focus on the development and application of bio-inspired materials/devices and functional tissue engineering models for elucidating regenerative biology, drug screening, disease modeling, and stem cell-based therapies.

  • Combinatorial maturation of iPSC derived skeletal muscle cell - nanotopography and mechanical stretching
  • iPSC myogenic differentiation
  • Tissue engineered human neuromuscular junctions for study of CMT II pathophysiology
  • Graphene-based multifunctional nanomaterials for cardiac tissue and stem cell Engineering
  • Biomimetically nano-textured micro electrode array device for drug-induced cardiotoxicity screening
  • Biocompatible transparent substrate for corneal epithelial cell migration enhancement
  • Mathematical modeling of transfer of intercellular cytoplasmic proteins
  • Thermoresponsive nanofabricated substratum for the engineering of three dimensional tissues with layer-by-layer architectural control
  • Synergistic effects of topography and rigidity difference on human umbilical smooth muscle cell
  • Paid part-time research scientist from Jan. 2012 to Aug. 2012
  • Multiscale device fabrication manager
  • Mentored undergraduate students and volunteers

Education

University of Washington

PhD Molecular Engineering
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Sep 2018 - Current

University of Washington

MS Applied Bioengineering
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Aug 2016 - Dec 2017

University of Washington

BS Physiology
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Aug 2010 - Jun 2016

Projects

Evaluation of the periodontal regenerative properties of patterned human periodontal ligament stem cell sheets

Journal of Periodontal & Implant Science
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2018

Junghyun Kim#, Sukyoung Ko#, Justin H. Lee, Min Soo Bae Deok-Ho Kim, Jeong- Ho Yun

Micro-and nano-patterned conductive graphene-PEG hybrid scaffolds for cardiac tissue engineering

Chemical Communications
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2017

Alec Smith, Hyok Yoo, Hyunjung Yi, Eun Hyun Ahn, Justin H. Lee, Guozheng Shao, Ekaterina Nagornyak, Michael Laflamme, Charles E. Murry, and Deok-Ho Kim

Physical transfer of cytoplasmic proteins through cell-cell contact can lead to spread of signaling proteins

Integrative Biology
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2015

Kshitiz, Junaid Afzal, Eun Hyun Ahn, Yasir Suhail, Ruchi Goyal, Maimon E. Hubbi, Qasim Hussaini, David D. Ellison, Jatinder Goyal, Benjamin Nacev, Deok-Ho Kim, Justin H. Lee, Sam Frankel, Rashmi Bankoti, Andy J. Chien, and Andre Levchenko,

Combined Effects of Substrate Topography and Stiffness on Endothelial Cytokine and Chemokine Secretion

ACS Applied Materials & Interfaces
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2015

Hyeona Jeon#, Sue Im Jang#, Jonathan H. Tsui, Justin H. Lee, Soojin Park, Deok-Ho Kim, and Yong Chool Boo

Modeling intercellular transfer of biomolecules through tunneling nanotubes

Bulletin of Mathematical Biology
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2013

Yasir Suhail#, Kshitiz#, Justin H. Lee, Mark Walker, Deok-Ho Kim, Joel Bader, and Andre Levchenko

Skills

  • Molecular Cloning and PCR
  • scRT-PCR
  • Multiphoton Microscopy
  • Confocal Microscopy
  • Prototyping (3D printing, CNC, laser cutting)
  • Stem Cell Maintenance and Differentiation
  • Western Blotting
  • Immumnofluroscence Staining
  • ImageJ
  • Soft Lithography